The Exchange Reaction of Peptides R-d-Alanyl-d-alanine with d-[14C]Alanine to R-d-Alanyl-d-[14C]alanine and d-Alanine, Catalysed by the Membranes of Streptococcus faecalis ATCC 9790
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چکیده
منابع مشابه
Complexation of acetyl-D-alanyl-D-alanine by antibiotic A35512B.
Antibiotic A3 5512B1.2) is a member of the glycopeptide family of antibiotics; complete structures have been reported for two members of this group, vancomycin3' and ristocetin A4' a) . Both vancomycin and ristocetin have been shown to form complexes with mucopeptides containing the terminal dipeptide D-alanyl-D-alaninee) ; this interaction within the bacterial cell inhibits crosslinking of the...
متن کاملMolecular interactions of a semisynthetic glycopeptide antibiotic with D-alanyl-D-alanine and D-alanyl-D-lactate residues.
LY191145 is an N-alkylated glycopeptide antibiotic (the p-chlorobenzyl derivative of LY264826) with activity against vancomycin-susceptible and -resistant bacteria. Similar to vancomycin, LY191145 inhibited polymerization of peptidoglycan when muramyl pentapeptide served as a substrate but not when muramyl tetrapeptide was used, signifying a substrate-dependent mechanism of inhibition. Examinat...
متن کاملKinetic evidence for the formation of D-alanyl phosphate in the mechanism of D-alanyl-D-alanine ligase.
The steady state kinetic mechanism, molecular isotope exchange and the positional isotope exchange (PIX) reactions of D-alanyl-D-alanine ligase from Salmonella typhimurium have been studied. The kinetic mechanism has been determined to be ordered Ter-Ter from initial velocity and product inhibition experiments. The first substrate to bind is ATP followed by the addition of 2 mol of D-alanine. P...
متن کاملActive-site-serine D-alanyl-D-alanine-cleaving-peptidase-catalysed acyl-transfer reactions
Under certain conditions, the values of the parameters that govern the interactions between the active-siteserine D-alanyl-D-alanine-cleaving peptidases and both carbonyl-donor substrates and ,J-lactam suicide substrates can be determined on the basis of the amounts of (serine ester-linked) acyl-protein formed during the reactions. Expressing the 'affinity' of a ,J-lactam compound for a DD-pept...
متن کاملModifications of the acyl-D-alanyl-D-alanine terminus affecting complex-formation with vancomycin.
Vancomycin forms complexes with peptides terminating in d-alanyl-d-alanine that are analogous to the biosynthetic precursors of bacterial mucopeptides. The specificity of complex-formation has been studied by means of many synthetic peptides, prepared by both solid-phase and conventional methods. The following conclusions can be drawn: (a) three amide linkages are required to form a stable comp...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1977
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1977.tb11521.x